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<blockquote data-quote="KJ" data-source="post: 69027" data-attributes="member: 1">[URL unfurl=&quot;true&quot;]https://pubmed.ncbi.nlm.nih.gov/32053214/[/URL]Results: The GEO database search identified a specific gene expression signature for osteo/odontogenic differentiation. Analysis using ssCMap found that acetylsalicylic acid [(ASA)/aspirin] was the drug with the strongest correlation with that gene signature. The treatment of DPCs with 0.05 mmol L-1 ASA showed increased alkaline phosphatase activity (P &lt; 0.001), mineralization (P &lt; 0.05), and increased the expression of the osteo/odontogenic genes, DMP1 and DSPP (P &lt; 0.05). Low concentration (0.05 mmol L-1 ) ASA reduced inflammatory cytokines IL-6 (P &lt; 0.001), CCL21 (P &lt; 0.05) and MMP-9 (P &lt; 0.05) in an ex vivo pulpitis model.Conclusions: Connectivity mapping, a web-based informatics method, was successfully used to identify aspirin as a candidate drug that could modulate the differentiation of DPCs. Aspirin was shown to induce odontogenic differentiation in DPCs in vitro and this, together with its anti-inflammatory effects, makes it a potential candidate for vital pulp therapies.</blockquote>

[QUOTE="KJ, post: 69027, member: 1"] [URL unfurl="true"]https://pubmed.ncbi.nlm.nih.gov/32053214/[/URL] [B]Results: [/B]The GEO database search identified a specific gene expression signature for osteo/odontogenic differentiation. Analysis using ssCMap found that acetylsalicylic acid [(ASA)/aspirin] was the drug with the strongest correlation with that gene signature. The treatment of DPCs with 0.05 mmol L-1 ASA showed increased alkaline phosphatase activity (P < 0.001), mineralization (P < 0.05), and increased the expression of the osteo/odontogenic genes, DMP1 and DSPP (P < 0.05). Low concentration (0.05 mmol L-1 ) ASA reduced inflammatory cytokines IL-6 (P < 0.001), CCL21 (P < 0.05) and MMP-9 (P < 0.05) in an ex vivo pulpitis model. [B]Conclusions: [/B]Connectivity mapping, a web-based informatics method, was successfully used to identify aspirin as a candidate drug that could modulate the differentiation of DPCs. [B]Aspirin was shown to induce odontogenic differentiation in DPCs in vitro and this, together with its anti-inflammatory effects, makes it a potential candidate for vital pulp therapies.[/B] [/QUOTE]

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